Yeast vitality test



Yeast vitality is another important factor for healthy fermentation and is an indicator of metabolic activity/health.  Viable yeast are not always vital and knowing this ahead of time can save an entire batch of beer.  Although there are many ways to measure yeast vitality, we use the Nexcelom X2 Cellometer to measure esterase enzymatic activity.  Samples are stained 1-to-1 with Carboxyfluorescein-AM fluorescent enzymatic stain for 45 minutes and then analyzed for vitality. Bright field images are used for total cell count and fluorescent images are used to measure the active yeast cells to get the percentage of cells that are vital.


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