Yeast viability test
Yeast viability (percentage of the population that is alive) is a critical parameter of a healthy fermentation and is typically measured using methylene blue and a hemacytometer. Because this method is highly tedious, error-prone, and time consuming, we measure yeast viability with the Nexcelom X2 Cellometer. This instrument is specifically optimized for simple, 1-step determination of yeast concentration and viability using acridine orange and propidium iodide. Performance of the Cellometer X2 has been proven in the largest breweries in the U.S. and is used to automate their fermentation monitoring. Bright field images can be viewed to confirm cell morphology. Counted bright field images show counted cells even within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both bright field and fluorescent images from each sample makes 1-step concentration and viability determination possible. Viability is measured in just 60 seconds giving you almost instant results to nail down that pitch rate or understand how fermentation is progressing.
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